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Histological and IHC staining for transmitting light microscopy. Images show (left to right) deposits within or around the warp thread, crossing points with high and low load of red blood cells (RBCs) and the interspace of the blood compartment; blood flow in z-direction. Optimal staining results were performed using ( A1–A4 ) hematoxylin eosin staining (HE) with 1.0% eosin (RBCs pink-red) and 6 min hemalum (nucleated cells blue) incubation mounted with <t>VectaMount®;</t> ( B1–B4 ) Pappenheim staining with 3 min May-Grunwald (nucleated cells blue) and Giemsa (RBCs pink) stainings mounted with Fluoromount-G™; IHC stainings using anti-MPO antibody at dilution 1:100 and visualization with ( C1–C4 ) Histogreen (MPO green) or ( D1–D4 ) DAB (MPO brown), both counterstained with hemalum (nucleated cells blue) and mounted with Entellan. The sections were slightly affected by the stains: (A) Eosin with VectaMount® AQ mounting led to a slight color change of PMP fibers to rose; (A,C) hemalum stained HistoCURE 8100 lightly gray-blue; (B) Pappenheim mounted with Fluoromount-G™ stained HistoCURE 8100 light blue and the PMP fibers light purple; (D) DAB stained all plastic components lightly yellow-brown.
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Histological and IHC staining for transmitting light microscopy. Images show (left to right) deposits within or around the warp thread, crossing points with high and low load of red blood cells (RBCs) and the interspace of the blood compartment; blood flow in z-direction. Optimal staining results were performed using ( A1–A4 ) hematoxylin eosin staining (HE) with 1.0% eosin (RBCs pink-red) and 6 min hemalum (nucleated cells blue) incubation mounted with <t>VectaMount®;</t> ( B1–B4 ) Pappenheim staining with 3 min May-Grunwald (nucleated cells blue) and Giemsa (RBCs pink) stainings mounted with Fluoromount-G™; IHC stainings using anti-MPO antibody at dilution 1:100 and visualization with ( C1–C4 ) Histogreen (MPO green) or ( D1–D4 ) DAB (MPO brown), both counterstained with hemalum (nucleated cells blue) and mounted with Entellan. The sections were slightly affected by the stains: (A) Eosin with VectaMount® AQ mounting led to a slight color change of PMP fibers to rose; (A,C) hemalum stained HistoCURE 8100 lightly gray-blue; (B) Pappenheim mounted with Fluoromount-G™ stained HistoCURE 8100 light blue and the PMP fibers light purple; (D) DAB stained all plastic components lightly yellow-brown.
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Histological and IHC staining for transmitting light microscopy. Images show (left to right) deposits within or around the warp thread, crossing points with high and low load of red blood cells (RBCs) and the interspace of the blood compartment; blood flow in z-direction. Optimal staining results were performed using ( A1–A4 ) hematoxylin eosin staining (HE) with 1.0% eosin (RBCs pink-red) and 6 min hemalum (nucleated cells blue) incubation mounted with VectaMount®; ( B1–B4 ) Pappenheim staining with 3 min May-Grunwald (nucleated cells blue) and Giemsa (RBCs pink) stainings mounted with Fluoromount-G™; IHC stainings using anti-MPO antibody at dilution 1:100 and visualization with ( C1–C4 ) Histogreen (MPO green) or ( D1–D4 ) DAB (MPO brown), both counterstained with hemalum (nucleated cells blue) and mounted with Entellan. The sections were slightly affected by the stains: (A) Eosin with VectaMount® AQ mounting led to a slight color change of PMP fibers to rose; (A,C) hemalum stained HistoCURE 8100 lightly gray-blue; (B) Pappenheim mounted with Fluoromount-G™ stained HistoCURE 8100 light blue and the PMP fibers light purple; (D) DAB stained all plastic components lightly yellow-brown.

Journal: Frontiers in Cardiovascular Medicine

Article Title: Polymer embedding of membrane lungs for histological investigations of intra-device clot formation

doi: 10.3389/fcvm.2026.1650978

Figure Lengend Snippet: Histological and IHC staining for transmitting light microscopy. Images show (left to right) deposits within or around the warp thread, crossing points with high and low load of red blood cells (RBCs) and the interspace of the blood compartment; blood flow in z-direction. Optimal staining results were performed using ( A1–A4 ) hematoxylin eosin staining (HE) with 1.0% eosin (RBCs pink-red) and 6 min hemalum (nucleated cells blue) incubation mounted with VectaMount®; ( B1–B4 ) Pappenheim staining with 3 min May-Grunwald (nucleated cells blue) and Giemsa (RBCs pink) stainings mounted with Fluoromount-G™; IHC stainings using anti-MPO antibody at dilution 1:100 and visualization with ( C1–C4 ) Histogreen (MPO green) or ( D1–D4 ) DAB (MPO brown), both counterstained with hemalum (nucleated cells blue) and mounted with Entellan. The sections were slightly affected by the stains: (A) Eosin with VectaMount® AQ mounting led to a slight color change of PMP fibers to rose; (A,C) hemalum stained HistoCURE 8100 lightly gray-blue; (B) Pappenheim mounted with Fluoromount-G™ stained HistoCURE 8100 light blue and the PMP fibers light purple; (D) DAB stained all plastic components lightly yellow-brown.

Article Snippet: 49 , VectaMount® AQ , H-5501, Vector Laboratories, Newark, CA, USA.

Techniques: Immunohistochemistry, Light Microscopy, Staining, Incubation